LDHB Knockout cell line (HeLa)

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LDHB Knockout cell line (HeLa) Order

LDHB Knockout cell line (HeLa)
Catalog#
YKO-HS7594
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LDHB Knockout cell line (HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LDHB Knockout cell line (HeLa)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
LDHB
Gene ID
3945
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 4 (based on transcript LDHB-201)
Validation: PCR+Sanger Sequencing
LDHB Knockout cell line (HeLa) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LDHB gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LDHB gene knockout LDHB Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LDHB Knockout cell line (HeLa)
LDHB Knockout cell line (HeLa)
Catalog#
YKO-HS7594
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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